Practical Enzymology

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The following has been excerpted from a very popular Worthington publication which was originally published in as the Manual of Clinical Enzyme Measurements. While some of the presentation may seem somewhat dated, the basic concepts are still helpful for researchers who must use enzymes but who have little background in enzymology. The use of enzymes in the diagnosis of disease is one of the important benefits derived from the intensive research in biochemistry since the 's.

Enzymes have provided the basis for the field of clinical chemistry. It is, however, only within the recent past few decades that interest in diagnostic enzymology has multiplied. Many methods currently on record in the literature are not in wide use, and there are still large areas of medical research in which the diagnostic potential of enzyme reactions has not been explored at all. This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.

Sir, Despite the enormous technological and scientific progress that biomedical sciences have witnessed over the last decades, the automation of the majority of laboratory techniques used for the conduction of routine assays for industrial, academic, and clinical purposes and the commercialization of a significant number of diagnostic tests into commercially-available kits, a significant percentage of the basic biomedical research output is still set up and conducted in a non-automatic, analytical bench-based biochemical manner.

Open in a separate window. Eisenthal R, Danson M. Oxford: Oxford University Press; Enzyme Assays: A Practical Approach. Measurement of enzyme activity. Methods Enzymol. Enzyme assays for high-throughput screening. Curr Opin Biotechnol. Recent advances in enzyme assays.

Trends Biotechnol. Woodley JM. Condition: New.

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